Hotspot

trnS-GCU-trnG-UCC

LSC · 8896-9627 · 732 bp

Back to Physospermopsis

Pi 0.024

MarkerSeek score 67.50

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 732
variable_sites: 43
pi: 0.024
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	GCTTTAGTCCACTCAGCCAT	GGGGGTTACTAGAACGAATCA	50.0	47.6	57.6	56.8	617-809 bp	1.0	67.8
trnS-GCU-trnG-UCC_p2	GCTTTAGTCCACTCAGCCAT	GGGGGTTACTAGAACGAATCAC	50.0	50.0	57.6	58.2	617-809 bp	1.0	67.5
trnS-GCU-trnG-UCC_p3	AGTCCACTCAGCCATCTCTC	GGGGGTTACTAGAACGAATCA	55.0	47.6	58.5	56.8	612-804 bp	1.0	67.0
trnS-GCU-trnG-UCC_p4	GCTTTAGTCCACTCAGCCAT	AGGGGGTTACTAGAACGAATCA	50.0	45.5	57.6	58.3	618-810 bp	1.0	66.9
trnS-GCU-trnG-UCC_p5	AGTCCACTCAGCCATCTCTC	GGGGGTTACTAGAACGAATCAC	55.0	50.0	58.5	58.2	612-804 bp	1.0	66.7