Hotspot

rps16-trnQ-UUG

LSC · 6313-7542 · 1230 bp

Back to Physospermopsis

Pi 0.0278
MarkerSeek score 70.90
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1230
variable_sites
76
pi
0.0278
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.96
markerseek_score
70.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA TCGGAGGTTCGAATCCTTTC 45.0 50.0 58.1 57.3 1304-1685 bp 1.0 81.5
rps16-trnQ-UUG_p2 CGGATCGTGTCCTTCAAGTC TCGGAGGTTCGAATCCTTTC 55.0 50.0 58.4 57.3 1319-1700 bp 1.0 79.6
rps16-trnQ-UUG_p3 GGATCGTGTCCTTCAAGTCG TCGGAGGTTCGAATCCTTTC 55.0 50.0 58.4 57.3 1318-1699 bp 1.0 79.6
rps16-trnQ-UUG_p4 CGTTGCTTTCTACCACATCG TCGGAGGTTCGAATCCTTTC 50.0 50.0 57.2 57.3 1296-1677 bp 1.0 76.3
rps16-trnQ-UUG_p5 AGTCGCACGTTGCTTTCTAC TCGGAGGTTCGAATCCTTTC 50.0 50.0 58.9 57.3 1303-1684 bp 1.0 75.7