Hotspot

trnK-UUU-rps16

LSC · 4292-5201 · 910 bp

Back to Physospermopsis

Pi 0.0244
MarkerSeek score 64.70
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
910
variable_sites
61
pi
0.0244
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.98
markerseek_score
64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCCTTTCAGGATCAGTCGTG TGCTGCCATTTTGGAAGGAT 55.0 45.0 58.0 58.1 1660-1715 bp 1.0 87.9
trnK-UUU-rps16_p2 CCTTTCAGGATCAGTCGTGG TGCTGCCATTTTGGAAGGAT 55.0 45.0 58.0 58.1 1659-1714 bp 1.0 87.9
trnK-UUU-rps16_p3 TCCCTCGCTTCATCCAAATG TGCTGCCATTTTGGAAGGAT 50.0 45.0 58.0 58.1 1608-1663 bp 1.0 87.8
trnK-UUU-rps16_p4 CCCTTTCAGGATCAGTCGTG GCCGTACGAGGAGAAAACTT 55.0 50.0 58.0 57.9 1246-1308 bp 1.0 87.5
trnK-UUU-rps16_p5 CCTTTCAGGATCAGTCGTGG GCCGTACGAGGAGAAAACTT 55.0 50.0 58.0 57.9 1245-1307 bp 1.0 87.5