Hotspot

trnH-GUG-psbA

LSC · 81-454 · 374 bp

Back to Physospermopsis

Pi 0.0591
MarkerSeek score 74.60
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
374
variable_sites
57
pi
0.0591
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.93
markerseek_score
74.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG AGCTCCATCTACAAATGGGT 55.0 45.0 58.1 56.2 395-421 bp 1.0 76.5
trnH-GUG-psbA_p2 CAATCCACTGCCTTGATCCA AGCTCCATCTACAAATGGGT 50.0 45.0 57.9 56.2 409-435 bp 1.0 76.5
trnH-GUG-psbA_p3 AATCCACTGCCTTGATCCAC AGCTCCATCTACAAATGGGT 50.0 45.0 57.9 56.2 408-434 bp 1.0 76.5
trnH-GUG-psbA_p4 ACAATCCACTGCCTTGATCC AGCTCCATCTACAAATGGGT 50.0 45.0 57.9 56.2 410-436 bp 1.0 76.5
trnH-GUG-psbA_p5 GATCCACTTGGCTACATCCG AAGCTCCATCTACAAATGGGT 55.0 42.9 58.1 57.0 396-422 bp 1.0 74.7