Hotspot

ycf1

SSC · 126174-131873 · 5700 bp

Back to Physochlaina

Pi 0.00294

MarkerSeek score 52.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5700
variable_sites: 25
pi: 0.00294
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GGGTAATCTAGAAATAAATGAGTCACT	AAACTGGCTAAGACGGCATT	33.3	45.0	56.9	57.8	304 bp	1.0	41.1
ycf1_p2	AGGGTAATCTAGAAATAAATGAGTCAC	AAAAACTGGCTAAGACGGCA	33.3	45.0	56.9	58.0	307 bp	1.0	41.0
ycf1_p3	GGGTAATCTAGAAATAAATGAGTCACT	AAAAACTGGCTAAGACGGCA	33.3	45.0	56.9	58.0	306 bp	1.0	41.0
ycf1_p4	AGGGTAATCTAGAAATAAATGAGTCAC	AAACTGGCTAAGACGGCATT	33.3	45.0	56.9	57.8	305 bp	1.0	41.0
ycf1_p5	GGGTAATCTAGAAATAAATGAGTCACT	AAAACTGGCTAAGACGGCAT	33.3	45.0	56.9	57.8	305 bp	1.0	41.0