Hotspot

ndhF-rpl32

SSC · 114669-115431 · 763 bp

Back to Physochlaina

Pi 0.00612
MarkerSeek score 52.50
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
763
variable_sites
7
pi
0.00612
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TGAAAGGTATGATCCACGCA TGATGTCGAAGTGCGTTTTT 45.0 40.0 56.9 56.6 836-838 bp 1.0 60.9
ndhF-rpl32_p2 TGAAAGGTATGATCCACGCA TTGATGTCGAAGTGCGTTTT 45.0 40.0 56.9 56.6 837-839 bp 1.0 60.9
ndhF-rpl32_p3 TGAAAGGTATGATCCACGCA TTTGATGTCGAAGTGCGTTT 45.0 40.0 56.9 56.6 838-840 bp 1.0 60.9
ndhF-rpl32_p4 TGAAAGGTATGATCCACGCA TTTTGATGTCGAAGTGCGTT 45.0 40.0 56.9 56.6 839-841 bp 1.0 60.9
ndhF-rpl32_p5 TGAAAGGTATGATCCACGCA TTTTTGATGTCGAAGTGCGT 45.0 40.0 56.9 56.6 840-842 bp 1.0 60.9