Hotspot

rpl36-rps8

LSC · 82059-82508 · 450 bp

Back to Physochlaina

Pi 0.0207

MarkerSeek score 59.00

Primer available yes

Label rpl36-rps8

Feature Metadata

label_name: rpl36-rps8
feature_type: igs
region: LSC
length_bp: 450
variable_sites: 14
pi: 0.0207
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-rps8_p1	GCCTACGGATTAGTCGACAT	GAGAGGCTCGACTAGAAGGA	50.0	55.0	56.9	57.7	561-569 bp	1.0	71.1
rpl36-rps8_p2	GCCTACGGATTAGTCGACAT	TCCGCGAATTTTAGGTGGAA	50.0	45.0	56.9	57.5	626-634 bp	1.0	69.7
rpl36-rps8_p3	GCCTACGGATTAGTCGACAT	TTCCGCGAATTTTAGGTGGA	50.0	45.0	56.9	57.5	627-635 bp	1.0	69.7
rpl36-rps8_p4	CGCCTACGGATTAGTCGACA	GAGAGGCTCGACTAGAAGGA	55.0	55.0	59.3	57.7	562-570 bp	1.0	69.0
rpl36-rps8_p5	GCCTACGGATTAGTCGACATT	GAGAGGCTCGACTAGAAGGA	47.6	55.0	57.6	57.7	561-569 bp	1.0	68.8