Hotspot

trnL-UAA-trnF-GAA

LSC · 49266-49627 · 362 bp

Back to Physochlaina

Pi 0.00737

MarkerSeek score 62.00

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: LSC
length_bp: 362
variable_sites: 4
pi: 0.00737
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	ACATGTCAATACCGGCAACA	TTTGAACTGGTGACACGAGG	45.0	50.0	57.8	58.1	506-522 bp	1.0	80.4
trnL-UAA-trnF-GAA_p2	ACATGTCAATACCGGCAACA	GTGCCAGGAACCAGATTTGA	45.0	50.0	57.8	58.1	521-537 bp	1.0	80.0
trnL-UAA-trnF-GAA_p3	ACATGTCAATACCGGCAACA	AATCATGTGCCAGGAACCAG	45.0	50.0	57.8	58.2	527-543 bp	1.0	79.5
trnL-UAA-trnF-GAA_p4	ACATGTCAATACCGGCAACA	CATGTGCCAGGAACCAGATT	45.0	50.0	57.8	58.2	524-540 bp	1.0	79.5
trnL-UAA-trnF-GAA_p5	AATCGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.5	58.1	447-463 bp	1.0	78.4