Hotspot

ycf3-trnS-GGA

LSC · 45719-46555 · 837 bp

Back to Physochlaina

Pi 0.00958
MarkerSeek score 65.10
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
837
variable_sites
12
pi
0.00958
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 TTCTCCTGAAGTTGTCGGAA GCGAAGGTACGGAAAGAGAG 45.0 55.0 56.4 58.1 1029-1042 bp 1.0 67.7
ycf3-trnS-GGA_p2 TTTCTCCTGAAGTTGTCGGA GCGAAGGTACGGAAAGAGAG 45.0 55.0 56.4 58.1 1030-1043 bp 1.0 67.7
ycf3-trnS-GGA_p3 TTCTCCTGAAGTTGTCGGAA CCCTCGGTAAACAAAAGCCT 45.0 50.0 56.4 58.1 1000-1013 bp 1.0 67.7
ycf3-trnS-GGA_p4 TTTCTCCTGAAGTTGTCGGA CCCTCGGTAAACAAAAGCCT 45.0 50.0 56.4 58.1 1001-1014 bp 1.0 67.7
ycf3-trnS-GGA_p5 TTCTCCTGAAGTTGTCGGAA ACGGAAAGAGAGGGATTCGA 45.0 50.0 56.4 58.2 1021-1034 bp 1.0 67.2