Hotspot

psaA-ycf3

LSC · 42933-43721 · 789 bp

Back to Physochlaina

Pi 0.00256

MarkerSeek score 62.20

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 789
variable_sites: 3
pi: 0.00256
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	CCCATTCCTCGAAAGACGTT	ATCAAGCCGCTGAGTATTGG	50.0	50.0	57.9	58.0	952-966 bp	1.0	80.3
psaA-ycf3_p2	CCCATTCCTCGAAAGACGTT	GGAAACAGGCTATAGCGCTT	50.0	50.0	57.9	58.0	934-948 bp	1.0	80.3
psaA-ycf3_p3	CCCATTCCTCGAAAGACGTT	AGCCGCTGAGTATTGGAAAC	50.0	50.0	57.9	58.3	948-962 bp	1.0	78.6
psaA-ycf3_p4	CCCATTCCTCGAAAGACGTT	CAAGCCGCTGAGTATTGGAA	50.0	50.0	57.9	58.3	950-964 bp	1.0	78.6
psaA-ycf3_p5	CCCATTCCTCGAAAGACGTT	TAGCGCTTACTCCCGGTAAT	50.0	50.0	57.9	58.3	922-936 bp	1.0	78.3