Hotspot

atpH-atpI

LSC · 13841-14982 · 1142 bp

Back to Physochlaina

Pi 0.00293

MarkerSeek score 60.90

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1142
variable_sites: 5
pi: 0.00293
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	GGCAGAAACCAGTGGATTCA	TACCTGTCATGCTCCTTGGA	50.0	50.0	58.1	58.0	1264-1272 bp	1.0	79.4
atpH-atpI_p2	TACCCTCTACAGCTTGACCC	TACCTGTCATGCTCCTTGGA	55.0	50.0	58.1	58.0	1358-1366 bp	1.0	79.0
atpH-atpI_p3	GCGATACCCTCTACAGCTTG	TACCTGTCATGCTCCTTGGA	55.0	50.0	57.9	58.0	1362-1370 bp	1.0	78.9
atpH-atpI_p4	AATAACGGAAGCGGCAGAAA	TACCTGTCATGCTCCTTGGA	45.0	50.0	58.2	58.0	1276-1284 bp	1.0	78.6
atpH-atpI_p5	GCCAATCCAGCAGCAATAAC	TACCTGTCATGCTCCTTGGA	50.0	50.0	57.8	58.0	1290-1298 bp	1.0	78.4