Hotspot

trnK-UUU-rps16

LSC · 4334-5025 · 692 bp

Back to Physochlaina

Pi 0.00771
MarkerSeek score 64.00
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
692
variable_sites
8
pi
0.00771
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT TTGAAAAAGGGGCTCAACCT 50.0 45.0 58.2 57.5 822-825 bp 1.0 75.9
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT TTGAAAAAGGGGCTCAACCT 50.0 45.0 58.2 57.5 821-824 bp 1.0 75.9
trnK-UUU-rps16_p3 TCAGTCGTGGTCTTCCAAAC TTGAAAAAGGGGCTCAACCT 50.0 45.0 57.8 57.5 907-910 bp 1.0 75.2
trnK-UUU-rps16_p4 CAGTCGTGGTCTTCCAAACT TTGAAAAAGGGGCTCAACCT 50.0 45.0 57.8 57.5 906-909 bp 1.0 75.2
trnK-UUU-rps16_p5 AAAAGACACTAGCCGCACTT TTGAAAAAGGGGCTCAACCT 45.0 45.0 57.7 57.5 843-846 bp 1.0 75.1