Hotspot

rpoC2

LSC · 16912-21225 · 4314 bp

Back to Physocarpus

Pi 0.000232

MarkerSeek score 53.70

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4314
variable_sites: 1
pi: 0.000232
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	CCGCGTGTACATTCCCATAA	AGATCACTTCGGAATGGCAT	50.0	45.0	58.1	57.0	200 bp	1.0	71.8
rpoC2_p2	TTCTACCGCGTGTACATTCC	AGATCACTTCGGAATGGCAT	50.0	45.0	57.7	57.0	205 bp	1.0	70.0
rpoC2_p3	CCGCGTGTACATTCCCATAA	TAGATCACTTCGGAATGGCA	50.0	45.0	58.1	56.3	201 bp	1.0	66.6
rpoC2_p4	CCGCGTGTACATTCCCATAA	ACATCACACATCCTGGATCA	50.0	45.0	58.1	56.2	178 bp	1.0	65.8
rpoC2_p5	CCGCGTGTACATTCCCATAA	GCCAATCTGGTCTTTCACAA	50.0	45.0	58.1	56.2	268 bp	1.0	65.7