Hotspot

rpl20

LSC · 71758-72111 · 354 bp

Back to Physocarpus

Pi 0

MarkerSeek score 34.80

Primer available yes

Label rpl20

Feature Metadata

label_name: rpl20
feature_type: gene
region: LSC
length_bp: 354
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 34.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20_p1	CCAGAGTTCATTCTCCAGGG	ACGGGAAGGGAATAGCTGAA	55.0	50.0	57.3	58.4	485 bp	1.0	71.0
rpl20_p2	CCCAGAGTTCATTCTCCAGG	ACGGGAAGGGAATAGCTGAA	55.0	50.0	57.3	58.4	486 bp	1.0	71.0
rpl20_p3	CCAGAGTTCATTCTCCAGGG	TAGACTAGGACACGGGAAGG	55.0	55.0	57.3	57.6	496 bp	1.0	70.9
rpl20_p4	CCCAGAGTTCATTCTCCAGG	TAGACTAGGACACGGGAAGG	55.0	55.0	57.3	57.6	497 bp	1.0	70.9
rpl20_p5	CCAGAGTTCATTCTCCAGGG	AGGACACGGGAAGGGAATAG	55.0	55.0	57.3	58.5	490 bp	1.0	70.3