Hotspot

rbcL

LSC · 58241-59668 · 1428 bp

Back to Physocarpus

Pi 0.0021

MarkerSeek score 60.00

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: LSC
length_bp: 1428
variable_sites: 3
pi: 0.0021
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	ATGAGTTGTAGGGAGGGACC	AGTAAAAGATTGGGCCGAGT	55.0	45.0	58.1	56.8	1515 bp	1.0	69.8
rbcL_p2	ATGAGTTGTAGGGAGGGACC	AGTAAAAGATTGGGCCGAGTT	55.0	42.9	58.1	57.6	1515 bp	1.0	67.7
rbcL_p3	ATGAGTTGTAGGGAGGGACC	GTAAAAGATTGGGCCGAGTT	55.0	45.0	58.1	56.0	1514 bp	1.0	63.4
rbcL_p4	TGATGAGTTGTAGGGAGGGA	AGTAAAAGATTGGGCCGAGT	50.0	45.0	57.1	56.8	1517 bp	1.0	63.2
rbcL_p5	ATGAGTTGTAGGGAGGGACC	GTAAAAGATTGGGCCGAGTTT	55.0	42.9	58.1	56.8	1514 bp	1.0	61.5