Hotspot

rpl32-trnL-UAG

SSC · 117384-118318 · 935 bp

Back to Physocarpus

Pi 0.00107
MarkerSeek score 59.40
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
935
variable_sites
1
pi
0.00107
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGATATCGGGCAGCATTGAA AGCGTGTCTACCGATTTCAC 50.0 50.0 58.1 58.0 1054-1055 bp 1.0 79.2
rpl32-trnL-UAG_p2 GGATATCGGGCAGCATTGAA CAGCGTGTCTACCGATTTCA 50.0 50.0 58.1 58.0 1055-1056 bp 1.0 79.2
rpl32-trnL-UAG_p3 GGGCAGCATTGAAAGCTTTT AGCGTGTCTACCGATTTCAC 45.0 50.0 57.8 58.0 1047-1048 bp 1.0 78.6
rpl32-trnL-UAG_p4 GGGCAGCATTGAAAGCTTTT CAGCGTGTCTACCGATTTCA 45.0 50.0 57.8 58.0 1048-1049 bp 1.0 78.6
rpl32-trnL-UAG_p5 GGATATCGGGCAGCATTGAA TCCTAAGAGCAGCGTGTCTA 50.0 50.0 58.1 57.9 1064-1065 bp 1.0 78.3