Hotspot

ycf3-trnS-GGA

LSC · 46823-47725 · 903 bp

Back to Physocarpus

Pi 0.00111

MarkerSeek score 59.40

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 903
variable_sites: 1
pi: 0.00111
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TGCCTCTTTTTCTCCCGAAG	ACGGAAAGAGAGGGATTCGA	50.0	50.0	57.8	58.2	1096 bp	1.0	77.4
ycf3-trnS-GGA_p2	GCCTCTTTTTCTCCCGAAGT	ACGGAAAGAGAGGGATTCGA	50.0	50.0	57.8	58.2	1095 bp	1.0	77.4
ycf3-trnS-GGA_p3	TGCCTCTTTTTCTCCCGAAG	TTCCAATGCTACGCCTTGAA	50.0	45.0	57.8	57.8	1046 bp	1.0	77.1
ycf3-trnS-GGA_p4	GCCTCTTTTTCTCCCGAAGT	TTCCAATGCTACGCCTTGAA	50.0	45.0	57.8	57.8	1045 bp	1.0	77.0
ycf3-trnS-GGA_p5	TGCCTCTTTTTCTCCCGAAG	AGTTCCAATGCTACGCCTTG	50.0	50.0	57.8	58.5	1048 bp	1.0	74.2