Hotspot

psbZ-trnG-GCC

LSC · 38269-38758 · 490 bp

Back to Physocarpus

Pi 0.00857
MarkerSeek score 65.90
Primer available yes
Label psbZ-trnG-GCC

Feature Metadata

label_name
psbZ-trnG-GCC
feature_type
igs
region
LSC
length_bp
490
variable_sites
4
pi
0.00857
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-GCC_p1 TTTGCTTCTCCTGAGGGTTG AATCGAACCCGCATCTTCTC 50.0 50.0 57.7 58.1 659-689 bp 1.0 80.5
psbZ-trnG-GCC_p2 TTTGCTTCTCCTGAGGGTTG GAATCGAACCCGCATCTTCT 50.0 50.0 57.7 58.1 660-690 bp 1.0 80.5
psbZ-trnG-GCC_p3 GGGTTGGTCGGGTAACAAAA AATCGAACCCGCATCTTCTC 50.0 50.0 58.3 58.1 645-675 bp 1.0 80.3
psbZ-trnG-GCC_p4 GGGTTGGTCGGGTAACAAAA GAATCGAACCCGCATCTTCT 50.0 50.0 58.3 58.1 646-676 bp 1.0 80.3
psbZ-trnG-GCC_p5 TTTGCTTCTCCTGAGGGTTG GAACCCGCATCTTCTCCTTG 50.0 55.0 57.7 58.6 655-685 bp 1.0 76.0