Hotspot

trnH-GUG-psbA

LSC · 251-528 · 278 bp

Back to Physocarpus

Pi 0

MarkerSeek score 31.40

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 278
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 31.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	GCGCTAACCTTGGTATGGAA	55.0	50.0	58.1	58.0	394 bp	1.0	78.7
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	408 bp	1.0	78.7
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	407 bp	1.0	78.7
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	409 bp	1.0	78.7
trnH-GUG-psbA_p5	TTATTATGGGCGAACGACGG	GCGCTAACCTTGGTATGGAA	50.0	50.0	58.1	58.0	455 bp	1.0	78.6