Hotspot

ndhI-ndhA

SSC · 119275-119358 · 84 bp

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Pi 0.0238

MarkerSeek score 62.60

Primer available yes

Label ndhI-ndhA

Feature Metadata

label_name: ndhI-ndhA
feature_type: igs
region: SSC
length_bp: 84
variable_sites: 2
pi: 0.0238
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI-ndhA_p1	CAGCTCGTAGGGTTTGTTGA	AGATGGACTTTACCTAGGCT	50.0	45.0	57.8	54.9	259 bp	1.0	54.6
ndhI-ndhA_p2	AGCTCGTAGGGTTTGTTGAC	AGATGGACTTTACCTAGGCT	50.0	45.0	57.8	54.9	258 bp	1.0	54.6
ndhI-ndhA_p3	CAGCTCGTAGGGTTTGTTGA	ACCTAGGCTAAGAATGGATCAAC	50.0	43.5	57.8	57.7	248 bp	1.0	53.0
ndhI-ndhA_p4	AGCTCGTAGGGTTTGTTGAC	ACCTAGGCTAAGAATGGATCAAC	50.0	43.5	57.8	57.7	247 bp	1.0	52.9
ndhI-ndhA_p5	CAGCTCGTAGGGTTTGTTGA	ACCTAGGCTAAGAATGGATCA	50.0	42.9	57.8	55.5	248 bp	1.0	51.6