Hotspot

ndhF-rpl32

SSC · 111867-112724 · 858 bp

Back to Physaria

Pi 0.0129
MarkerSeek score 66.50
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
858
variable_sites
11
pi
0.0129
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 AGGTACCGGAAGTGGAATGA ACGATGTCCAATATCCCTTCC 50.0 47.6 58.1 57.2 1000-1004 bp 1.0 66.5
ndhF-rpl32_p2 ACCGGAAGTGGAATGAAAGG ACGATGTCCAATATCCCTTCC 50.0 47.6 57.8 57.2 996-1000 bp 1.0 65.3
ndhF-rpl32_p3 AGGTACCGGAAGTGGAATGA CGATGTCCAATATCCCTTCCT 50.0 47.6 58.1 56.9 999-1003 bp 1.0 64.2
ndhF-rpl32_p4 AGGTACCGGAAGTGGAATGA AACGATGTCCAATATCCCTTCC 50.0 45.5 58.1 57.9 1001-1005 bp 1.0 64.0
ndhF-rpl32_p5 AGGTACCGGAAGTGGAATGA AAGCTTTCAACGATGTCCAA 50.0 40.0 58.1 55.9 1009-1013 bp 1.0 63.7