Hotspot

rps12-clpP

LSC · 68742-68924 · 183 bp

Back to Physaria

Pi 0.0383

MarkerSeek score 70.90

Primer available yes

Label rps12-clpP

Feature Metadata

label_name: rps12-clpP
feature_type: igs
region: LSC
length_bp: 183
variable_sites: 7
pi: 0.0383
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-clpP_p1	TGACATTTCGGATTGGCTGT	TATGTCAGCAACAGAAGCCC	45.0	50.0	57.8	57.9	296 bp	1.0	79.4
rps12-clpP_p2	CGAGTACATGTTCCTCGTCG	TATGTCAGCAACAGAAGCCC	55.0	50.0	58.2	57.9	348 bp	1.0	79.2
rps12-clpP_p3	CCGAGTACATGTTCCTCGTC	TATGTCAGCAACAGAAGCCC	55.0	50.0	57.8	57.9	349 bp	1.0	79.2
rps12-clpP_p4	TTTCGGATTGGCTGTCTTGT	TATGTCAGCAACAGAAGCCC	45.0	50.0	57.7	57.9	291 bp	1.0	78.9
rps12-clpP_p5	CACCCGAGTACATGTTCCTC	TATGTCAGCAACAGAAGCCC	55.0	50.0	57.7	57.9	352 bp	1.0	78.3