Hotspot

accD-psaI

LSC · 58091-58758 · 668 bp

Back to Physaria

Pi 0.00605
MarkerSeek score 52.40
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
LSC
length_bp
668
variable_sites
4
pi
0.00605
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
52.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 TGAGTTATTTCAGCTCCATGC GACATTGCAATTGCCGGAAA 42.9 45.0 56.3 57.9 790-797 bp 1.0 59.0
accD-psaI_p2 TGAGTTATTTCAGCTCCATGC ACATTGCAATTGCCGGAAAG 42.9 45.0 56.3 57.9 789-796 bp 1.0 58.9
accD-psaI_p3 TGAGTTATTTCAGCTCCATGC TGCAATTGCCGGAAAGACTA 42.9 45.0 56.3 57.8 785-792 bp 1.0 58.1
accD-psaI_p4 TGAGTTATTTCAGCTCCATGC CCGGAAAGACTAGGCCTACT 42.9 55.0 56.3 58.2 777-784 bp 1.0 57.8
accD-psaI_p5 TGAGTTATTTCAGCTCCATGC CATTGCAATTGCCGGAAAGA 42.9 45.0 56.3 57.6 788-795 bp 1.0 56.7