Hotspot

trnL-trnF

LSC · 47298-47893 · 596 bp

Back to Physaria

Pi 0.00839

MarkerSeek score 63.50

Primer available yes

Label trnL-trnF

Feature Metadata

label_name: trnL-trnF
feature_type: igs
region: LSC
length_bp: 596
variable_sites: 5
pi: 0.00839
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-trnF_p1	AATCGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.5	58.1	686-890 bp	1.0	76.3
trnL-trnF_p2	AATCGTGAGGGTTCAAGTCC	GAACTGGTGACACGAGGATT	50.0	50.0	57.5	57.5	683-887 bp	1.0	73.1
trnL-trnF_p3	TTCAAGTCCCTCTATCCCCA	TTTGAACTGGTGACACGAGG	50.0	50.0	57.0	58.1	675-879 bp	1.0	72.4
trnL-trnF_p4	AATCGTGAGGGTTCAAGTCC	TACCAACTGAGCTATCCCGG	50.0	55.0	57.5	58.6	648-852 bp	1.0	72.0
trnL-trnF_p5	AATCGTGAGGGTTCAAGTCC	TGAACTGGTGACACGAGGAT	50.0	50.0	57.5	58.7	684-888 bp	1.0	71.4