Hotspot

trnD-trnY

LSC · 29763-30185 · 423 bp

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Pi 0.0143

MarkerSeek score 63.70

Primer available yes

Label trnD-trnY

Feature Metadata

label_name: trnD-trnY
feature_type: igs
region: LSC
length_bp: 423
variable_sites: 6
pi: 0.0143
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-trnY_p1	CGGTGCTCTGACCAATTGAA	TGGCAATATGTCTACGCTGG	50.0	50.0	58.5	57.8	491-493 bp	1.0	75.6
trnD-trnY_p2	CGGTGCTCTGACCAATTGAA	GGCAATATGTCTACGCTGGT	50.0	50.0	58.5	57.8	490-492 bp	1.0	75.6
trnD-trnY_p3	CGGTGCTCTGACCAATTGAA	GGGGACGGACTGTAAATTCG	50.0	55.0	58.5	58.4	512-514 bp	1.0	74.6
trnD-trnY_p4	CGGTGCTCTGACCAATTGAA	GGGACGGACTGTAAATTCGT	50.0	50.0	58.5	57.6	511-513 bp	1.0	74.4
trnD-trnY_p5	CGGTGCTCTGACCAATTGAA	GATTCTTCCTGGGTCGATGC	50.0	55.0	58.5	58.4	545-547 bp	1.0	74.1