Hotspot

petN-psbM

LSC · 28035-28561 · 527 bp

Back to Physaria

Pi 0.00775

MarkerSeek score 63.70

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 527
variable_sites: 4
pi: 0.00775
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	CGTGGGCTGCTTTAATGGTA	TGCTACTGCACTCTTCATTCT	50.0	42.9	58.3	57.0	666-677 bp	1.0	64.0
petN-psbM_p2	CGTGGGCTGCTTTAATGGTA	TGCACTCTTCATTCTTGTTCCT	50.0	40.9	58.3	57.9	660-671 bp	1.0	63.0
petN-psbM_p3	TAGTGTGGGGAAGAAGTGGA	TGCTACTGCACTCTTCATTCT	50.0	42.9	57.6	57.0	621-632 bp	1.0	62.9
petN-psbM_p4	GTAGTGTGGGGAAGAAGTGG	TGCTACTGCACTCTTCATTCT	55.0	42.9	57.5	57.0	622-633 bp	1.0	62.4
petN-psbM_p5	TAGTGTGGGGAAGAAGTGGA	TGCACTCTTCATTCTTGTTCCT	50.0	40.9	57.6	57.9	615-626 bp	1.0	61.9