Hotspot

trnQ-psbK

LSC · 6538-6867 · 330 bp

Back to Physaria

Pi 0.0152
MarkerSeek score 61.80
Primer available yes
Label trnQ-psbK

Feature Metadata

label_name
trnQ-psbK
feature_type
igs
region
LSC
length_bp
330
variable_sites
5
pi
0.0152
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnQ-psbK_p1 AACTGTACTCTGGGACGGAA TGCGACTAAAAAGGTGCTTG 50.0 45.0 58.0 56.9 483 bp 1.0 72.3
trnQ-psbK_p2 AACTGTACTCTGGGACGGAA ACGATTGGATTCAAAAAGGCG 50.0 42.9 58.0 57.8 521 bp 1.0 71.0
trnQ-psbK_p3 AACTGTACTCTGGGACGGAA CGATTGGATTCAAAAAGGCGT 50.0 42.9 58.0 57.8 520 bp 1.0 71.0
trnQ-psbK_p4 GTACTCTGGGACGGAAGGAT TGCGACTAAAAAGGTGCTTG 55.0 45.0 58.2 56.9 479 bp 1.0 70.6
trnQ-psbK_p5 AACTGTACTCTGGGACGGAA GCGACTAAAAAGGTGCTTGA 50.0 45.0 58.0 56.7 482 bp 1.0 70.0