Hotspot

ycf1

SSC · 126545-132271 · 5727 bp

Back to Physalis

Pi 0.0091

MarkerSeek score 54.40

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5727
variable_sites: 187
pi: 0.0091
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGCCCAATTTCCAGATTCAGT	TCTAAACTGTACCCCTCCCG	42.9	55.0	57.8	58.1	453-501 bp	0.866667	71.1
ycf1_p2	TGCCCAATTTCCAGATTCAGT	TCCCGTGGAGAGCTAATGAA	42.9	50.0	57.8	58.1	438-486 bp	0.866667	71.0
ycf1_p3	TGCCCAATTTCCAGATTCAGT	GAAGCCTGGGATAGCATTCC	42.9	55.0	57.8	58.1	753-801 bp	0.866667	69.4
ycf1_p4	TGCCCAATTTCCAGATTCAGT	GGATAGCATTCCTTTTGCGC	42.9	50.0	57.8	57.9	745-793 bp	0.866667	69.3
ycf1_p5	TGCCCAATTTCCAGATTCAGT	GCCTGGGATAGCATTCCTTT	42.9	50.0	57.8	57.6	750-798 bp	0.866667	67.3