Hotspot

rpl2

LSC · 87121-88611 · 1491 bp

Back to Physalis

Pi 0.00695

MarkerSeek score 50.50

Primer available yes

Label rpl2

Feature Metadata

label_name: rpl2
feature_type: gene
region: LSC
length_bp: 1491
variable_sites: 26
pi: 0.00695
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2_p1	ACGTGTCACAAGCTTACTCC	CGCTTCAACCGGGTTATTCT	50.0	50.0	57.8	58.3	1638-1643 bp	1.0	78.4
rpl2_p2	ACGTGTCACAAGCTTACTCC	ATCATTACGCTTCAACCGGG	50.0	50.0	57.8	58.3	1645-1650 bp	1.0	77.9
rpl2_p3	ACGTGTCACAAGCTTACTCC	ACGCTTCAACCGGGTTATTC	50.0	50.0	57.8	58.6	1639-1644 bp	1.0	76.1
rpl2_p4	CGTGTCACAAGCTTACTCCT	CGCTTCAACCGGGTTATTCT	50.0	50.0	57.6	58.3	1637-1642 bp	1.0	76.1
rpl2_p5	CGTGTCACAAGCTTACTCCT	ATCATTACGCTTCAACCGGG	50.0	50.0	57.6	58.3	1644-1649 bp	1.0	75.5