Hotspot

clpP

LSC · 72909-74912 · 2004 bp

Back to Physalis

Pi 0.00388

MarkerSeek score 56.40

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2004
variable_sites: 26
pi: 0.00388
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	AGTTGGCATGTTGAATCGTA	ACGTTTCCAACTCAAAGTGA	40.0	40.0	55.4	55.4	2166-2186 bp	1.0	41.2
clpP_p2	TAGTTGGCATGTTGAATCGT	ACGTTTCCAACTCAAAGTGA	40.0	40.0	55.4	55.4	2167-2187 bp	1.0	41.2
clpP_p3	AGTTGGCATGTTGAATCGTA	ACGTTTCCAACTCAAAGTGAA	40.0	38.1	55.4	56.2	2166-2186 bp	1.0	41.2
clpP_p4	TAGTTGGCATGTTGAATCGT	ACGTTTCCAACTCAAAGTGAA	40.0	38.1	55.4	56.2	2167-2187 bp	1.0	41.2
clpP_p5	TGGGTTGGTTTAGATTGATCCT	ACGTTTCCAACTCAAAGTGA	40.9	40.0	57.1	55.4	2134-2154 bp	1.0	41.2