Hotspot

rpl32-trnL-UAG

SSC · 115847-116822 · 976 bp

Back to Physalis

Pi 0.00391

MarkerSeek score 58.90

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 976
variable_sites: 16
pi: 0.00391
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AGGAAGGGATATTGGGTAGCA	TCCTAAGAGCAGCGTGTCTA	47.6	50.0	57.9	57.9	1088-1120 bp	1.0	75.6
rpl32-trnL-UAG_p2	AGGAAGGGATATTGGGTAGCA	TCGAACCGAGATGCATTAGC	47.6	50.0	57.9	58.1	1114-1146 bp	1.0	75.4
rpl32-trnL-UAG_p3	AGGAAGGGATATTGGGTAGCA	AGCAGCGTGTCTACCAATTT	47.6	45.0	57.9	57.8	1081-1113 bp	1.0	75.2
rpl32-trnL-UAG_p4	AGGAAGGGATATTGGGTAGCA	CCTAAGAGCAGCGTGTCTAC	47.6	55.0	57.9	57.8	1087-1119 bp	1.0	75.1
rpl32-trnL-UAG_p5	AGGAAGGGATATTGGGTAGCA	CTAAGAGCAGCGTGTCTACC	47.6	55.0	57.9	57.8	1086-1118 bp	1.0	75.1