Hotspot

rps19-rpl2

LSC · 87050-87120 · 71 bp

Back to Physalis

Pi 0.0131

MarkerSeek score 57.40

Primer available yes

Label rps19-rpl2

Feature Metadata

label_name: rps19-rpl2
feature_type: igs
region: LSC
length_bp: 71
variable_sites: 7
pi: 0.0131
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-rpl2_p1	GCCCGGGACCAAGTTACTAT	TTGATTCTTCGTCGCCGTAG	55.0	50.0	59.2	58.1	207-212 bp	1.0	74.1
rps19-rpl2_p2	GCCCGGGACCAAGTTACTAT	CACAACCCCTTGGGGTTATC	55.0	55.0	59.2	58.2	274-279 bp	1.0	72.6
rps19-rpl2_p3	GCCCGGGACCAAGTTACTAT	ATTTGATTCTTCGTCGCCGT	55.0	45.0	59.2	58.3	209-214 bp	1.0	72.6
rps19-rpl2_p4	GCCCGGGACCAAGTTACTAT	TTGGGGTTATCCTGCACTTG	55.0	50.0	59.2	57.8	265-270 bp	1.0	72.3
rps19-rpl2_p5	CCCGGGACCAAGTTACTATT	TTGATTCTTCGTCGCCGTAG	50.0	50.0	56.6	58.1	206-211 bp	1.0	72.3