Hotspot

ndhC-trnV-UAC

LSC · 52731-53865 · 1135 bp

Back to Physalis

Pi 0.00279

MarkerSeek score 55.90

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1135
variable_sites: 16
pi: 0.00279
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 55.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	CTCCGGAAATGAAAAATGCCA	AGAAGGTCTACGGTTCGAGT	42.9	50.0	57.4	57.8	1206-1267 bp	1.0	70.2
ndhC-trnV-UAC_p2	ACTCCGGAAATGAAAAATGCC	AGAAGGTCTACGGTTCGAGT	42.9	50.0	57.4	57.8	1207-1268 bp	1.0	70.1
ndhC-trnV-UAC_p3	TCCGGAAATGAAAAATGCCA	AGAAGGTCTACGGTTCGAGT	40.0	50.0	56.2	57.8	1205-1266 bp	1.0	68.5
ndhC-trnV-UAC_p4	CTCCGGAAATGAAAAATGCCA	GAAGGTCTACGGTTCGAGTC	42.9	55.0	57.4	57.5	1205-1266 bp	1.0	67.4
ndhC-trnV-UAC_p5	ACTCCGGAAATGAAAAATGCC	GAAGGTCTACGGTTCGAGTC	42.9	55.0	57.4	57.5	1206-1267 bp	1.0	67.4