Hotspot

trnH-GUG-psbA

LSC · 132-571 · 440 bp

Back to Physalis

Pi 0.0287

MarkerSeek score 73.60

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 440
variable_sites: 46
pi: 0.0287
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	TCGAAGCTCCATCTACAAATGG	55.0	45.5	58.1	58.2	480-512 bp	0.866667	67.4
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	TCGAAGCTCCATCTACAAATGG	50.0	45.5	57.9	58.2	494-526 bp	0.866667	67.2
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	TCGAAGCTCCATCTACAAATGG	50.0	45.5	57.9	58.2	493-525 bp	0.866667	67.2
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	TCGAAGCTCCATCTACAAATGG	50.0	45.5	57.9	58.2	495-527 bp	0.866667	67.2
trnH-GUG-psbA_p5	GATCCACTTGGCTACATCCG	CGAAGCTCCATCTACAAATGG	55.0	47.6	58.1	56.8	479-511 bp	0.866667	67.2