Hotspot

rpoA

LSC · 80818-81831 · 1014 bp

Back to Physaliastrum

Pi 0.00197

MarkerSeek score 59.90

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: LSC
length_bp: 1014
variable_sites: 2
pi: 0.00197
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	TCAAAGCGAATTCTCCCTAGA	AATGGCTGTAGACCTCCGAA	42.9	50.0	56.5	58.4	1235 bp	1.0	56.4
rpoA_p2	TCAAAGCGAATTCTCCCTAGA	ATGGCTGTAGACCTCCGAAA	42.9	50.0	56.5	58.4	1234 bp	1.0	56.4
rpoA_p3	TCAAAGCGAATTCTCCCTAGA	TAATGGCTGTAGACCTCCGA	42.9	50.0	56.5	57.6	1236 bp	1.0	56.2
rpoA_p4	TCAAAGCGAATTCTCCCTAGA	GGCTGTAGACCTCCGAAAAA	42.9	50.0	56.5	57.5	1232 bp	1.0	56.1
rpoA_p5	TCAAAGCGAATTCTCCCTAGA	TGGCTGTAGACCTCCGAAAA	42.9	50.0	56.5	58.7	1233 bp	1.0	54.6