Hotspot

petD

LSC · 79412-80638 · 1227 bp

Back to Physaliastrum

Pi 0.00163

MarkerSeek score 59.70

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1227
variable_sites: 2
pi: 0.00163
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	TCATGTCAACTAAACGGGGG	AGGGAGAATTCGCTTTGAAGA	50.0	42.9	57.5	57.3	1358 bp	1.0	62.6
petD_p2	TCATGTCAACTAAACGGGGG	AGGGAGAATTCGCTTTGAAGAA	50.0	40.9	57.5	58.0	1358 bp	1.0	60.1
petD_p3	TCATGTCAACTAAACGGGGG	GGGAGAATTCGCTTTGAAGA	50.0	45.0	57.5	55.8	1357 bp	1.0	58.3
petD_p4	TCATGTCAACTAAACGGGGG	AGGGAGAATTCGCTTTGAAG	50.0	45.0	57.5	55.7	1358 bp	1.0	58.2
petD_p5	TTCATGTCAACTAAACGGGGG	AGGGAGAATTCGCTTTGAAGA	47.6	42.9	58.2	57.3	1359 bp	1.0	56.8