Hotspot

psbE-petL

LSC · 67579-68588 · 1010 bp

Back to Physaliastrum

Pi 0.00297

MarkerSeek score 60.60

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1010
variable_sites: 3
pi: 0.00297
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	TGACAAATAACCAACCCGCA	GTCGTATCTTGCTCAGACCA	45.0	50.0	58.0	57.1	1212-1213 bp	1.0	72.6
psbE-petL_p2	TGACAAATAACCAACCCGCA	AGCCGCTAATAGAAAACCGA	45.0	45.0	58.0	57.0	1166-1167 bp	1.0	72.3
psbE-petL_p3	CAACCCGCAATGAATAGGGA	GTCGTATCTTGCTCAGACCA	50.0	50.0	57.9	57.1	1201-1202 bp	1.0	72.3
psbE-petL_p4	CAACCCGCAATGAATAGGGA	AGCCGCTAATAGAAAACCGA	50.0	45.0	57.9	57.0	1155-1156 bp	1.0	72.1
psbE-petL_p5	GTTCTCCTGTGCTTCCAGAC	GTCGTATCTTGCTCAGACCA	55.0	50.0	58.2	57.1	1119-1120 bp	1.0	71.2