Hotspot

trnC-GCA-petN

LSC · 28836-29501 · 666 bp

Back to Physaliastrum

Pi 0.003

MarkerSeek score 60.50

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 666
variable_sites: 2
pi: 0.003
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	ATTTGTAGCATTTTGGCGGC	TTAAAGCAGCCCAAGCAAGA	45.0	45.0	58.0	58.0	786 bp	1.0	80.1
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.1	58.0	729 bp	1.0	79.5
trnC-GCA-petN_p3	ATTTGTAGCATTTTGGCGGC	CAGCCCAAGCAAGACTTACT	45.0	50.0	58.0	57.8	780 bp	1.0	78.6
trnC-GCA-petN_p4	ATTTGTAGCATTTTGGCGGC	CCTAGAGTCCACTCCTTCCC	45.0	60.0	58.0	58.2	843 bp	1.0	78.4
trnC-GCA-petN_p5	ATTTGTAGCATTTTGGCGGC	CTAGAGTCCACTCCTTCCCC	45.0	60.0	58.0	58.2	842 bp	1.0	78.4