Hotspot

trnR-UCU-atpA

LSC · 10330-10448 · 119 bp

Back to Physaliastrum

Pi 0.0168

MarkerSeek score 62.20

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 119
variable_sites: 2
pi: 0.0168
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	GGTTCAAATCCTATTGGACGC	CGAGGAAGCAGAAGTCCTTT	47.6	50.0	57.3	57.5	217 bp	1.0	63.5
trnR-UCU-atpA_p2	GGTTCAAATCCTATTGGACGC	TCAGGAACAAATGGACCGTT	47.6	45.0	57.3	57.4	184 bp	1.0	62.1
trnR-UCU-atpA_p3	GGTTCAAATCCTATTGGACGC	TTCAGGAACAAATGGACCGT	47.6	45.0	57.3	57.4	185 bp	1.0	62.1
trnR-UCU-atpA_p4	GGTTCAAATCCTATTGGACGC	TACCGAGGAAGCAGAAGTCC	47.6	55.0	57.3	58.8	220 bp	1.0	60.7
trnR-UCU-atpA_p5	TGGATAGGACAGAGGTCTTCT	CGAGGAAGCAGAAGTCCTTT	47.6	50.0	56.9	57.5	252 bp	1.0	60.5