Hotspot

rbcL-psaI

LSC · 58399-59687 · 1289 bp

Back to Phyllostachys

Pi 0.00321

MarkerSeek score 41.80

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 1289
variable_sites: 9
pi: 0.00321
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	AAATTTGAGTTCGAGCCGGT	CCATTGCAATTGCCGGAAAT	45.0	45.0	58.1	58.0	1397-1434 bp	1.0	79.5
rbcL-psaI_p2	GCCGCAGCTTGTGAAATATG	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.2	58.0	1427-1464 bp	1.0	79.0
rbcL-psaI_p3	AAATTTGAGTTCGAGCCGGT	AAGAAGCCATTGCAATTGCC	45.0	45.0	58.1	57.9	1403-1440 bp	1.0	78.9
rbcL-psaI_p4	GCCGCAGCTTGTGAAATATG	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.2	57.9	1433-1470 bp	1.0	78.4
rbcL-psaI_p5	CAAATTTGAGTTCGAGCCGG	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.8	58.0	1398-1435 bp	1.0	78.4