Hotspot

atpI-atpH

LSC · 33738-34552 · 815 bp

Back to Phyllostachys

Pi 0

MarkerSeek score 27.00

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: LSC
length_bp: 815
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 27.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	TAGCCGCAGCCTATATAGGT	AAGCAGCAGCAATTAGTGGA	50.0	45.0	57.5	57.8	884 bp	1.0	74.1
atpI-atpH_p2	ATTTTTGCAACGTTAGCCGC	AAGCAGCAGCAATTAGTGGA	45.0	45.0	58.6	57.8	897 bp	1.0	73.7
atpI-atpH_p3	TAGCCGCAGCCTATATAGGT	AGCAGCAGCAATTAGTGGAT	50.0	45.0	57.5	57.6	883 bp	1.0	72.3
atpI-atpH_p4	AGTCCCTATACCGGTCATGT	AAGCAGCAGCAATTAGTGGA	50.0	45.0	57.2	57.8	952 bp	1.0	72.2
atpI-atpH_p5	ACAAGCGGTATTCAAGCTCT	AAGCAGCAGCAATTAGTGGA	45.0	45.0	57.2	57.8	918 bp	1.0	72.2