Hotspot

matK-trnK-UUU

LSC · 3232-3926 · 695 bp

Back to Phyllostachys

Pi 0.00061
MarkerSeek score 42.30
Primer available yes
Label matK-trnK-UUU

Feature Metadata

label_name
matK-trnK-UUU
feature_type
igs
region
LSC
length_bp
695
variable_sites
3
pi
0.00061
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
42.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK-trnK-UUU_p1 ACTCCTGAAAGAGAAGTGGGT CGTGCGGTGTAATTCCATTG 47.6 50.0 58.3 57.8 861-865 bp 1.0 68.8
matK-trnK-UUU_p2 ACTCCTGAAAGAGAAGTGGG CGTGCGGTGTAATTCCATTG 50.0 50.0 56.5 57.8 861-865 bp 1.0 67.0
matK-trnK-UUU_p3 CTCCTGAAAGAGAAGTGGGT CGTGCGGTGTAATTCCATTG 50.0 50.0 56.5 57.8 860-864 bp 1.0 67.0
matK-trnK-UUU_p4 ACTCCTGAAAGAGAAGTGGGT TGGGTTGCTAACTCAATGGT 47.6 45.0 58.3 57.0 802-806 bp 1.0 62.5
matK-trnK-UUU_p5 ACTCCTGAAAGAGAAGTGGGTA CGTGCGGTGTAATTCCATTG 45.5 50.0 58.1 57.8 861-865 bp 1.0 62.1