Hotspot

ycf1

SSC · 125267-130930 · 5664 bp

Back to Phyllanthus

Pi 0.0608

MarkerSeek score 76.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5664
variable_sites: 878
pi: 0.0608
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AAAAGGCAGTTCAGCTTCCA	AAATGAAGAAAACGCGGCAG	45.0	45.0	57.9	57.9	856-862 bp	0.5	63.4
ycf1_p2	GAACCAAAAGGCAGTTCAGC	AAATGAAGAAAACGCGGCAG	50.0	45.0	57.9	57.9	861-867 bp	0.5	62.9
ycf1_p3	AAAAGGCAGTTCAGCTTCCA	GCAATTCTAGCGGATCCCAA	45.0	50.0	57.9	58.0	987 bp	0.166667	45.8
ycf1_p4	GAACCAAAAGGCAGTTCAGC	GCAATTCTAGCGGATCCCAA	50.0	50.0	57.9	58.0	992 bp	0.166667	45.3
ycf1_p5	AAGTGAAAGTCGTCTTCGGG	GCAATTCTAGCGGATCCCAA	50.0	50.0	57.8	58.0	1014 bp	0.166667	45.2