Hotspot

ndhF

SSC · 111436-113667 · 2232 bp

Back to Phyllanthus

Pi 0.0486

MarkerSeek score 77.30

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2232
variable_sites: 281
pi: 0.0486
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TCGTTGTGGTCGGACTCTAT	CCTTTGATGCTATGGAAGGGT	50.0	47.6	58.2	57.7	2793-2935 bp	0.333333	50.0
ndhF_p2	ATCAATTCGGTCGTTGTGGT	CCTTTGATGCTATGGAAGGGT	45.0	47.6	57.8	57.7	2803-2945 bp	0.333333	50.0
ndhF_p3	CGGGACAGCTCATAACACTC	CCTTTGATGCTATGGAAGGGT	55.0	47.6	58.1	57.7	2803 bp	0.166667	35.7
ndhF_p4	GGGCCCTCTTATCTCTTCCT	CCTTTGATGCTATGGAAGGGT	55.0	47.6	57.9	57.7	2888 bp	0.166667	35.4
ndhF_p5	TGGGGCCCTCTTATCTCTTC	CCTTTGATGCTATGGAAGGGT	55.0	47.6	58.2	57.7	2890 bp	0.166667	34.7