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clpP-psbB

LSC · 73632-74073 · 442 bp

Back to Phyllanthus

Pi 0.0523

MarkerSeek score 82.70

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 442
variable_sites: 57
pi: 0.0523
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	TTTGGAACACCAATAGGCAT	CGATACCAAGGCAAACCCAT	40.0	50.0	55.2	58.2	471-496 bp	1.0	67.6
clpP-psbB_p2	TTTGGAACACCAATAGGCAT	ACACGATACCAAGGCAAACC	40.0	50.0	55.2	58.5	474-499 bp	1.0	65.8
clpP-psbB_p3	TTTGGAACACCAATAGGCAT	TGAACACGATACCAAGGCAA	40.0	45.0	55.2	57.4	477-502 bp	1.0	65.1
clpP-psbB_p4	TTTGGAACACCAATAGGCAT	ATGAACACGATACCAAGGCA	40.0	45.0	55.2	57.2	478-503 bp	1.0	63.3
clpP-psbB_p5	TTTGGAACACCAATAGGCAT	ATGAACACGATACCAAGGCAA	40.0	42.9	55.2	57.9	478-503 bp	1.0	61.0