Hotspot

petA-psbJ

LSC · 64485-65460 · 976 bp

Back to Phyllanthus

Pi 0.0694

MarkerSeek score 83.80

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 976
variable_sites: 162
pi: 0.0694
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 83.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TCCCGCTACTAGGAAGGATG	ACACAATCAAACCCGAACGA	55.0	45.0	58.0	58.0	1745-1951 bp	0.833333	84.0
petA-psbJ_p2	TCCCGCTACTAGGAAGGATG	TTAGGAGGCCTCAATGACCA	55.0	50.0	58.0	58.0	1907-2113 bp	0.833333	83.7
petA-psbJ_p3	CCTGATCCCGCTACTAGGAA	TTAGGAGGCCTCAATGACCA	55.0	50.0	58.0	58.0	1912-2099 bp	0.833333	83.4
petA-psbJ_p4	TGATCCCGCTACTAGGAAGG	ACACAATCAAACCCGAACGA	55.0	45.0	58.0	58.0	1748-1928 bp	0.666667	75.5
petA-psbJ_p5	TGATCCCGCTACTAGGAAGG	TTAGGAGGCCTCAATGACCA	55.0	50.0	58.0	58.0	1910-2090 bp	0.666667	75.4