Hotspot

psaA-ycf3

LSC · 43431-44107 · 677 bp

Back to Phyllanthus

Pi 0.0446

MarkerSeek score 82.50

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 677
variable_sites: 81
pi: 0.0446
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	CTTCTGGTTCCGGCGAAC	TGGTTGAAAATCACAAGGCG	61.1	45.0	58.4	57.2	724-763 bp	1.0	64.6
psaA-ycf3_p2	CTTCTGGTTCCGGCGAAC	GGTTGAAAATCACAAGGCGT	61.1	45.0	58.4	57.2	723-762 bp	1.0	64.6
psaA-ycf3_p3	CTTCTGGTTCCGGCGAAC	TTGGTTGAAAATCACAAGGCG	61.1	42.9	58.4	57.9	725-764 bp	1.0	62.2
psaA-ycf3_p4	CTTCTGGTTCCGGCGAAC	GGTTGAAAATCACAAGGCGTT	61.1	42.9	58.4	57.9	723-762 bp	1.0	62.2
psaA-ycf3_p5	ACTTCTGGTTCCGGCGAA	TGGTTGAAAATCACAAGGCG	55.6	45.0	58.9	57.2	725-764 bp	1.0	61.3