Hotspot

trnG-GCC-trnM

LSC · 38110-38288 · 179 bp

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Pi 0.0566
MarkerSeek score 82.70
Primer available yes
Label trnG-GCC-trnM

Feature Metadata

label_name
trnG-GCC-trnM
feature_type
igs
region
LSC
length_bp
179
variable_sites
26
pi
0.0566
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.98
markerseek_score
82.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-trnM_p1 TCTTTGCCAAGGAGAAGACG CTTGAGGTCACGGGTTCAAA 50.0 50.0 57.8 58.0 240-257 bp 1.0 90.3
trnG-GCC-trnM_p2 TCTTTGCCAAGGAGAAGACG TGAGGTCACGGGTTCAAATC 50.0 50.0 57.8 57.8 238-255 bp 1.0 89.2
trnG-GCC-trnM_p3 TCTTTGCCAAGGAGAAGACG GTAGCTCGCAAGGCTCATAA 50.0 50.0 57.8 57.8 261-278 bp 1.0 88.5
trnG-GCC-trnM_p4 TCTTTGCCAAGGAGAAGACG TAGCTCGCAAGGCTCATAAC 50.0 50.0 57.8 57.8 260-277 bp 1.0 88.5
trnG-GCC-trnM_p5 TCTTTGCCAAGGAGAAGACG GAGGTCACGGGTTCAAATCC 50.0 55.0 57.8 58.5 237-254 bp 1.0 86.2