Hotspot

psbZ-trnG-GCC

LSC · 37305-38038 · 734 bp

Back to Phyllanthus

Pi 0.0663

MarkerSeek score 80.30

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 734
variable_sites: 108
pi: 0.0663
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	TTTTCTGGCTCGGCTATTCC	CTTGAGGTCACGGGTTCAAA	50.0	50.0	58.0	58.0	1558-1743 bp	0.833333	84.7
psbZ-trnG-GCC_p2	TTTTCTGGCTCGGCTATTCC	CCGAGAGCTAACTACCGAGA	50.0	55.0	58.0	58.0	1854-2057 bp	0.833333	84.2
psbZ-trnG-GCC_p3	TTTTCTGGCTCGGCTATTCC	CGTCTCCGCAACATTGAAAC	50.0	50.0	58.0	58.0	1535-1703 bp	0.666667	77.3
psbZ-trnG-GCC_p4	TTTTCTGGCTCGGCTATTCC	GGGGCGTATTCAATCCTTGT	50.0	50.0	58.0	57.9	1734-1836 bp	0.666667	75.8
psbZ-trnG-GCC_p5	TTTTCTGGCTCGGCTATTCC	ATGGTAGAGGGGCGTATTCA	50.0	50.0	58.0	57.9	1659-1844 bp	0.666667	75.8