Hotspot

trnE-UUC-trnT-GGU

LSC · 31416-32421 · 1006 bp

Back to Phyllanthus

Pi 0.0502

MarkerSeek score 84.40

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 1006
variable_sites: 124
pi: 0.0502
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 84.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	GGATTTGGATCTGTCGGGAC	GAACCGATGACTTACGCCTT	55.0	50.0	58.0	58.0	1569-1850 bp	1.0	91.6
trnE-UUC-trnT-GGU_p2	GGATTTGGATCTGTCGGGAC	CCATGGCGTTACTCTACCAC	55.0	55.0	58.0	58.1	1729-1829 bp	0.833333	83.2
trnE-UUC-trnT-GGU_p3	ATAACAGAAGCACGCATCGT	GAACCGATGACTTACGCCTT	45.0	50.0	58.0	58.0	1577-1627 bp	0.5	67.0
trnE-UUC-trnT-GGU_p4	ATAACAGAAGCACGCATCGT	CCATGGCGTTACTCTACCAC	45.0	55.0	58.0	58.1	1556-1606 bp	0.5	66.6
trnE-UUC-trnT-GGU_p5	ATAACAGAAGCACGCATCGT	TGGCGTTACTCTACCACTGA	45.0	50.0	58.0	58.1	1553-1603 bp	0.5	66.5